Elucidating altered transcriptional programs sedating newborns
Furthermore, gene expression data contains valuable directional information indicated by arrows next to the gene expression data utilized by URA (blue), which incorporates hierarchical systems biology networks.The core analysis of the workflow includes multi-omics data integration between chromatin binding and differential gene expression events The combination of both transcriptomic and epigenomic profiling offers insight into different levels of gene regulation, transcription factor binding motifs, DNA and chromatin modifications, and how each component is coupled to a functional output.Disruption of patterns of histone modifications is associated with the loss of proliferative control and cancer.There is tremendous therapeutic potential in understanding and targeting histone modification pathways.c By elucidating transcription factors associated with an epigenomic event or regulator, it is possible to identify a well-defined epigenomic-transcriptomic cooperation network supported by complementary multi-omics data.A color scheme denoting, both data types and systems biology analyses, is maintained throughout the entire document.
Epigenetics is the study of phenotypic changes that are not mediated through mutations in the DNA sequence.Thus, investigating cooperation of chromatin remodelers and the transcriptional machinery is not only important for elucidating fundamental mechanisms of chromatin regulation, but also necessary for the design of targeted therapeutics.Beyond genomic alterations, aberrant epigenomes contribute to many cancers, as demonstrated by widespread changes to DNA methylation patterns, redistribution of histone marks, and disruption of chromatin structure.The intersection of analysis of motif enrichment (AME) and transcription factor target (TFT), and upstream regulator analysis (URA) approaches provides insights into cooperative networks of transcription factors associated with epigenomic regulators.Importantly, such genome-wide information can be accessed at the sequence or gene level providing different level of depth and resolution.
Search for elucidating altered transcriptional programs:
Gene promoter annotation in combination with network analysis and sequence-resolution of enriched transcriptional motifs in epigenomic data reveals transcription factor families that act synergistically with epigenomic master regulators.